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选用二棱大麦14个基因型的未成熟胚进行离体培养,获得了全部基因型的再生植株,对其自交后代(R_2)的性状变异进行了研究,结果表明,14个基因型叶绿素缺失的变异频率平均为2.62％,以浅绿类型为主;10个基因型抽穗期和株高的变异频率平均分别为7.61％和6.51％,大部分为晚抽穗和矮秆变异,不同基因型间性状的变异频率有显著差异,如叶绿素缺失变异频率从1.02％到10.33％,抽穗期从2.09％到20.46％,株高从1.88％到13.43％;而不同基因型在叶绿素缺失的类型及抽穗期和株高的变异趋向上亦有完全不同的表现,说明基因型对大麦体细胞无性系二代性状的变异频率与趋向均有明显的影响。
In this study, 14 genotypes of two barley cultivars were used for in vitro culture. The regenerated plants of all genotypes were obtained and the trait variation of their selfing offspring (R_2) was studied. The results showed that 14 genotypes chlorophyll deficient The average frequency of variation was 2.62%, which was mainly of light green type. The average frequencies of variation of heading date and plant height of the 10 genotypes were 7.61% and 6.51% respectively, most of them were late heading and dwarf variation. The frequency of variation of chlorophyll was from 1.02% to 10.33%, heading from 2.09% to 20.46%, and plant height from 1.88% to 13.43%. The differences of chlorophyll deletion types and heading date The trend of variation in plant height also showed completely different behavior, indicating that the genotypes had a significant effect on the frequency and tendency of the second generation traits of somaclonal.2.