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目的：探讨维生素Ｄ受体（ＶＤＲ）基因Ｂ／ｂ多态性与慢性肾功能衰竭（ＣＲＦ）继发性甲状旁腺功能亢进的关系。方法：慢性肾衰患者１０８例，分为二组①高全段甲状旁腺素（ｉＰＴＨ）组：６１例，ｉＰＴＨ＞６０ｐｍｏｌ／Ｌ；②低ｉＰＴＨ组：４７例，ｉＰＴＨ＜１２ｐｍｏｌ／Ｌ。正常健康对照５９例。采用酚氯仿法提取外周血ＤＮＡ，多聚酶链反应法扩增ＶＤＲ基因第８内含子目的片段，ＢｓｍＩ酶消化ＰＣＲ产物，经２％琼脂糖溴乙锭凝胶电泳，紫外灯下观察结果。结果：维生素Ｄ基因型的分布在正常人群为ＢＢ型９例（１５２％），Ｂｂ型２６例（４４１％），ｂｂ型２４例（４０７％）；在慢性肾衰高ｉＰＴＨ组ＢＢ型６例（９８％），Ｂｂ型２２例（３６１％），ｂｂ型３３例（５４１％）；低ｉＰＴＨ组ＢＢ型１１例（２３４％），Ｂｂ型２０例（４２６％），ｂｂ型１６例（３４０％）。正常人群与ＣＲＦ患者ＢＢ、Ｂｂ、ｂｂ基因型及Ｂ、ｂ等位基因分布无明显差异（Ｐ＞００５）；而ＣＲＦ高ｉＰＴＨ组与低ｉＰＴＨ组相比，ＢＢ基因型和Ｂ等位基因的频率明显减低（Ｐ＜００５）；ｂｂ基因型和ｂ等位基因的频率明显升高（Ｐ＜００５）。结论：中国汉族ＣＲＦ患者不同ｉＰＴ?
Objective: To investigate the relationship between vitamin B receptor (VDR) gene B / b polymorphism and secondary hyperparathyroidism in chronic renal failure (CRF). Methods: One hundred and eight patients with chronic renal failure were divided into two groups: ① high total parathyroid hormone (iPTH) group: 61 cases, iPTH> 60 pmol / L; ② low iPTH group: 47 cases and iPTH <12 pmol / L. 59 cases of normal healthy control. Peripheral blood DNA was extracted by phenol-chloroform method. The target fragment of exon 8 of VDR gene was amplified by polymerase chain reaction. The PCR product was digested by BsmI enzyme and observed under UV light by 2% agarose ethidium bromide gel electrophoresis. Results: There were 9 cases (152%) of BB genotype, 26 cases (441%) of Bb genotype and 24 cases (407%) of bb genotype in the normal population. In the patients with chronic renal failure There were 6 cases (98%) in BB genotype in iPTH group, 22 cases (361%) in Bb genotype, 33 cases (541%) in bb type, 11 cases (234%) in BB genotype in low iPTH group, 20 cases (426%), bb type 16 cases (340%). There was no significant difference in genotypes of BB, Bb, bb and B and B alleles between normal subjects and patients with CRF (P> 0.05), while BB genotypes and B alleles of CRP high iPTH group and low iPTH group (P <005). The frequencies of bb genotype and b allele were significantly increased (P <005). Conclusion: Chinese Han CRF patients with different iPT?