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目的 :研究以党参、黄芪、何首乌、山萸肉等益肾健脑、益气养血为主方的中医药对切割海马伞大鼠学习、记忆力的影响以及对隔区胆碱能神经元的保护作用。方法 :实验组和对照组动物切割海马伞前后分别用中药煎剂和自来水灌胃 ,术后第 3、4周行避暗回避试验和跳台试验 ,最后灌注固定动物 ,取隔区切片作 Ch AT免疫组化和 NOS组化染色 ,显微镜下记数切割海马伞侧内侧隔核和斜角带垂直支 Ch AT和 NOS阳性神经元数 ,并观察胞体和纤维变化。结果 :避暗回避试验的探索次数、滞留时间及跳台试验的主动和总回避反应阳性率实验组均好于对照组 (P<0 .0 1或 0 .0 5 ) ,切割海马伞侧隔区 Ch AT和 NOS阳性神经元数均较对照组多 (P均 <0 .0 1) ,其胞体和纤维萎缩程度也较对照组轻。结论 :以党参、黄芪、何首乌、山萸肉等为主方的中医药对切割海马伞大鼠的学习、记忆及隔区的胆碱能神经元具有保护作用
OBJECTIVE: To study the effects of traditional Chinese medicinal herbs such as Dangshen, Radix Astragali, Radix Polygoni Multiflori, and Hawthorn Meat on the learning and memory of rats with hippocampal fimbriactriculus incision, and to cholinergic neurons in septal area. Protective effects. Methods: Animals of experimental group and control group were treated with traditional Chinese medicine decoction and tap water before and after the hippocampal umbrella was cut. After 3 and 4 weeks of operation, dark avoidance test and platform test were performed. Finally, animals were perfused and fixed. Immunohistochemistry and NOS staining were performed. The numbers of ChAT and NOS positive neurons in the medial septal nucleus and oblique band of the hippocampal side of the hippocampus were counted under a microscope, and cell body and fiber changes were observed. RESULTS: The number of explorations, residence time, and active and total avoidance responses of the dark avoidance test were better in the experimental group than in the control group (P < 0.01 or 0.05). The numbers of ChAT and NOS positive neurons were more than those in the control group (P<0.01), and the cell body and fiber atrophy were also lighter than those in the control group. Conclusion : Traditional Chinese medicine with Dangshen, Radix Astragali, Radix Polygoni Multiflori, and Hawthorn Meat as the main prescriptions has protective effects on learning, memory, and cholinergic neurons in the septal region of rats.