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近年来微生物细胞由于尺寸小、几何外形标准多样和资源丰富等特点成为制备金属纳米结构或材料极具吸引力的生物模板.以典型的革兰氏阴性菌——大肠杆菌为模板通过无电子化学镀成功制备了具有标准杆状外形的表面镀镍的纳米金属材料,并利用原子力显微镜和透射电子显微镜对化学镀镍前后的大肠杆菌作了形貌学上的表征、测量与对比.结果显示,空白对照菌表面平整光滑并在云母基底表面表现出较显著的铺展效应;经过活化和化学镀镍后的菌细胞表面粗糙度明显增大,并在基本保持原有菌模板外形的基础上,其高宽比有所增大.超薄切片结果表明,菌细胞表面的金属镍镀层分布均匀,其厚度处于纳米量级.
In recent years, due to its small size, diverse geometrical shapes and rich resources, microbial cells have become attractive bio templates for the preparation of metal nanostructures or materials.A typical template of Gram-negative bacteria, Escherichia coli, The surface-plated nickel-plated nano-metallic materials with a standard rod shape were successfully prepared and the morphological characterization, measurement and comparison of E. coli before and after electroless nickel plating were carried out by using atomic force microscope and transmission electron microscope.The results showed that, The surface of the blank control bacteria was smooth and smooth, and showed a remarkable spreading effect on the surface of the mica substrate. The surface roughness of the bacterial cells increased obviously after activation and electroless nickel plating. On the basis of keeping the shape of the original template, The aspect ratio increased.The results of ultrathin sections showed that the distribution of metallic nickel coating on the surface of bacterial cells was uniform and the thickness was on the order of nanometers.