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[目的]研究复方黄根对大鼠慢性肝损伤的保护作用及可能机制。[方法]制备大鼠四氯化碳(CCl4)慢性肝损伤模型,观察复方黄根对肝损伤大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST)活性,总蛋白(TP)、清蛋白(Alb)和羟脯氨酸(Hyp)水平的变化以及肝组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)水平的影响,放免法检测透明质酸(HA)、Ⅲ型前胶原肽(PⅢP)水平,免疫组化法测肝组织转化生长因子-β1(TGF-β1)的表达,并观察肝组织病理学改变。[结果]复方黄根可显著降低CCl4所致大鼠慢性肝损伤血清中ALT、AST、HA、PⅢP、Hyp水平,升高血清中Alb、TP水平;升高肝组织中SOD、GSH-Px的活性,并可降低MDA的水平;免疫组化结果表明复方黄根能抑制TGF-β1表达;病理观察结果能减轻慢性肝损伤的肝脏损伤程度。[结论]复方黄根有明显的保肝和抗肝纤维化作用,其作用机制可能与抗脂质过氧化和抑制肝组织TGF-β1的表达有关。
[Objective] To study the protective effect of compound Huanggen on chronic liver injury in rats and its possible mechanism. [Methods] Rat model of chronic liver injury induced by carbon tetrachloride (CCl4) was established. The activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum of rats with liver injury were observed. Changes in levels of (TP), albumin (Alb), and hydroxyproline (Hyp), and liver tissue homogenates superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase ( The level of GSH-Px) was measured by radioimmunoassay for the levels of hyaluronic acid (HA) and type III procollagen peptide (PIIIP). The expression of transforming growth factor-β1 (TGF-β1) in liver tissue was measured by immunohistochemistry and observed. Histopathological changes in the liver. [Results] Compound Huanggen could significantly reduce the levels of ALT, AST, HA, PIIIP, and Hyp in serum of chronic liver injury rats induced by CCl4, increase the levels of Alb and TP in serum, and increase the levels of SOD and GSH-Px in liver tissues. Activity, and can reduce the level of MDA; immunohistochemistry results showed that compound yellow root can inhibit the expression of TGF-β1; pathological observations can reduce the degree of liver injury in chronic liver injury. [Conclusion] The compound yellow root has obvious protective effect on liver and anti-hepatic fibrosis, and its mechanism may be related to anti-lipid peroxidation and inhibiting the expression of TGF-β1 in liver tissue.