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１９９６～１９９７年连续二年进行了固定氮素用量，改变铵态、硝态氮肥的施用比例试验，对烤烟的一些光合生理特性进行了研究。试验结果表明，在烤烟生长前、中期，增施铵态氮施用比例，烤烟的叶绿素含量增加，但达１００％铵态氮时，叶绿素含量反而下降，低于施用５０％和７５％铵态氮的处理；在烤烟生长后期，功能叶片叶绿素含量基本上与施用铵态氮比例高低一致，以１００％铵态氮处理的叶绿素含量最高。另外增加铵态氮施用比例，烤烟功能叶片的希尔反应活性及光合磷酸化活性有所提高，但Ｐ／Ｏ比值基本不变。从叶绿素诱导荧光动力学特征参数及比值情况来看：增加铵态氮施用比例，Ｆｍ和Ｆｖ值升高；几种荧光参数的比值如Ｆｄ／Ｆｓ、Ｆｖ／Ｆｏ、Ｆｖ／Ｆｍ比值有所上升，但均以２５％硝态氮＋７５％铵态氮的处理最高；并且其烤后烟叶的产量最高、品质最好。
From 1996 to 1997, nitrogen fixation was applied for two consecutive years to change the proportion of ammonium and nitrate nitrogen fertilizers. Some photosynthetic characteristics of flue-cured tobacco were studied. The results showed that the chlorophyll content of flue-cured tobacco increased with the proportion of ammonium nitrogen applied before and during the growth of flue-cured tobacco, but decreased with the increase of 100% ammonium nitrogen, but lower than that with 50% and 75% ammonium nitrogen The content of chlorophyll in functional leaves was basically the same with the proportion of ammonium nitrogen applied at the later stage of flue-cured tobacco growth, and the content of chlorophyll was the highest when treated with 100% ammonium nitrogen. In addition, increasing the proportion of ammonium nitrogen application, Hill reaction activity and photosynthetic phosphorylation activity of functional leaves increased, but the P / O ratio remained unchanged. From the chlorophyll fluorescence induced kinetic parameters and ratios, the ratio of Fm and Fv increased with the increase of ammonium nitrogen application rate. The ratios of several fluorescence parameters such as Fd / Fs, Fv / Fo and Fv / Fm increased , But the treatment with 25% nitrate nitrogen + 75% ammonium nitrogen was the highest, and the yield of the cured tobacco leaf was the highest with the best quality.