论文部分内容阅读
构建了一个水稻核内小分子RNA的cDNA文库,经初步筛选获得30种boxC/D snoRNA.与目前已鉴定的水稻snoRNA序列相比较,除了U14等7种snoRNA之外,其余23种均为首次在水稻中发现.在23种新的水稻boxC/D snoRNA中,11种只存在于水稻中,其余12种中,6种为植物特有,6种在酵母、动物和拟南芥中存在同源分子.17种snoRNA指导了水稻5.8S,18S和25S rRNA中24个2＇-O-核糖甲基化修饰核苷,其中19个靶位点的修饰已被证实.6种新的水稻snoRNA不具有与rRNA互补的反义序列,是一类新的snoRNA.研究结果表明,通过构建核内小分子RNA的cDNA文库可以有效地分离和鉴定水稻中新的snoRNA.这些新发现的snoRNA对于阐明植物snoRNA基因组织和表达以及rRNA中2＇-0-核糖甲基化修饰位点的产生机制具有重要意义.
A cDNA library of small RNAs in rice nucleus was constructed and 30 kinds of boxC / D snoRNAs were obtained through preliminary screening.Compared with the currently identified rice snoRNA sequences, 23 kinds of snoRNAs were the first Of the 23 new rice boxC / D snoRNAs, 11 are present in rice only, and of the remaining 12, 6 are plant-specific and 6 are homologous in yeast, animal and Arabidopsis The 17 snoRNAs direct the 24 2’-O-ribose methylated nucleosides in 5.8S, 18S and 25S rRNA of rice, of which 19 target sites have been confirmed. Six new rice snoRNAs It is a new type of snoRNA with antisense sequence complementary to rRNA.The results show that the new snoRNA in rice can be efficiently isolated and identified by constructing a cDNA library of small nuclear RNAs.These newly discovered snoRNAs are of great importance for elucidating the potential of plant The mechanisms by which the 2’-0-ribosylation site of snoRNAs are expressed and expressed in rRNA are important.