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目的探讨密度梯度离心法优选精液行IUI的临床疗效。方法采用密度梯度离心法制备精液行IUI 22个周期,并对制备前后的精液进行常规分析、改良巴氏染色、混合抗球蛋白反应实验。结果洗涤后的精子,其密度、活力、精子正常形态比率明显升高,精子畸形指数及精子包被抗体阳性率明显下降。结论密度梯度离心法优选精液行IUI可增加受孕机率。
Objective To investigate the clinical efficacy of density gradient centrifugation in optimizing semen IUI. Methods 22 cycles of semen IUI were prepared by density gradient centrifugation. The semen samples were routinely analyzed before and after semen preparation, and the modified pasteurized and mixed antiglobulin reaction experiments were performed. Results After washing, the sperm density, vitality, the normal morphology of sperm were significantly increased, the sperm deformity index and the positive rate of sperm coated antibody decreased significantly. Conclusion Density gradient centrifugation optimized sperm row IUI can increase the chance of conception.