采用枯草杆菌黑色芽孢变种作为实验菌种,介绍了菌液的配制、发菌系统原理、沉降菌和浮游菌的采样方法、细菌的培养与计数方法,以及隔离病房回风过滤器对细菌过滤效果检测、病人微生物气溶胶污染范围测定、隔离病房开关门隔离效果测定等的具体实验方法。 Bacillus subtilis black spore mutants were used as experimental strains. The preparation of bacterial liquid, the principle of germ-killing system, the sampling method of sedimentation bacteria and planktonic bacteria, the method of bacterial culture and counting, and the bacterial filtration effect of the isolation ward air filter were introduced Testing, the scope of the patient’s microbial aerosol contamination, isolation ward door isolation test, and other specific experimental methods.