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目的 :探讨人肺癌组织细胞自发凋亡的发生 ,p5 3基因及凋亡相关基因bcl 2、Bax在肺癌组织中的表达及与肺癌细胞凋亡和生物学行为的关系。方法 :用甲基绿 派诺宁染色检测 5 0例肺癌组织切片中凋亡细胞 ,LSAB免疫组化法检测 p5 3和Bax的蛋白表达 ,原位杂交方法检测bcl 2mRNA的表达。结果 :10 0 %小细胞肺癌凋亡发生数 10个 /HPF ,2 1%的肺鳞癌和 15 %的肺腺癌凋亡发生数 >10个 /HPF。p5 3与bcl 2的表达与肺癌淋巴结转移之间有显著统计意义 (P <0 0 5 ) ,p5 3的表达与bcl 2和Bax表达之间无统计学显著意义。结论 :小细胞肺癌较肺鳞癌和肺腺癌更易于发生自发凋亡 ,肺癌组织中 p5 3表达的改变并不一定伴有bcl 2和Bax表达的改变
Objective: To investigate the occurrence of spontaneous apoptosis in human lung cancer tissues, the expression of p53 gene and apoptosis-related genes bcl 2 and Bax in lung cancer tissues, and the relationship with apoptosis and biological behavior of lung cancer cells. METHODS: Apoptotic cells were detected in 50 lung cancer specimens using methyl green nononin staining. The expression of p53 and Bax proteins was detected by LSAB immunohistochemistry and bcl 2 mRNA expression was detected by in situ hybridization. Results : 100% of small cell lung cancer cells had 10/HPF apoptosis, 21% of lung squamous cell carcinoma and 15% of lung adenocarcinoma cells had apoptosis >10/HPF. There was significant statistical significance between the expression of p53 and bcl-2 and lymph node metastasis in lung cancer (P < 0.05). There was no statistically significant difference between the expression of p53 and bcl-2 and Bax. Conclusion :Small cell lung cancer is more prone to spontaneous apoptosis than lung squamous cell carcinoma and lung adenocarcinoma. The expression of p53 in lung cancer is not necessarily accompanied by changes in the expression of bcl 2 and Bax.