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目的:GPR54是下丘脑神经元调控下丘脑-垂体-性腺轴功能的门控,本文研究不同浓度脂多糖(LPS)、肿瘤坏死因子α(TNFα)、白细胞介素6(IL-6)、地塞米松和胰岛素对人乳腺癌MCF7细胞GPR54 mRNA及蛋白表达的影响。方法:培养MCF7细胞,用LPS(10μg/ml和20μg/ml)、TNFα(20 ng/ml和100 ng/ml)、IL-6(10 ng/ml和20 ng/ml)、地塞米松(10-6mol/L和10-7mol/L)和胰岛素(0.01 IU/L和0.1 IU/L)干预72 h,评价干预6、24、48、72 h后GPR54 mRNA(实时荧光定量PCR)和蛋白表达量(Western印迹)的变化。结果:和对照组相比,LPS(10μg/ml和20μg/ml)、TNFα(20 ng/ml和100 ng/ml)、IL-6(10 ng/ml和20 ng/ml)、地塞米松(10-6mol/L和10-7mol/L)和胰岛素(0.01 IU/L和0.1 IU/L)均显著增加GPR54 mRNA(P均<0.05)和蛋白表达。结论:LPS、TNFα、IL-6、地塞米松和胰岛素显著增加MCF7细胞GPR54的表达,提示这些炎症因子和激素可能通过调节GPR54水平变化进而影响性腺轴功能。
PURPOSE: GPR54 is the gating of hypothalamic-pituitary-gonadal axis regulated by hypothalamic neurons. The effects of different concentrations of lipopolysaccharide (LPS), tumor necrosis factor α (TNFα), interleukin 6 (IL-6) Effect of Dexamethasone and Insulin on GPR54 mRNA and Protein Expression in Human Breast Cancer MCF7 Cells. METHODS: MCF7 cells were cultured and treated with LPS (10 μg / ml and 20 μg / ml), TNFα (20 ng / ml and 100 ng / ml), IL-6 The mRNA and protein levels of GPR54 mRNA and protein were determined after intervention for 6, 24, 48 and 72 h by intervention of 10-6 mol / L and 10-7 mol / L insulin and 0.01 IU / L and 0.1 IU / Changes in expression (Western blot). Results: Compared with control group, LPS (10μg / ml and 20μg / ml), TNFα (20 ng / ml and 100 ng / ml), IL-6 (10-6mol / L and 10-7mol / L) and insulin (0.01 IU / L and 0.1 IU / L) significantly increased the expression of GPR54 mRNA (all P <0.05) and protein. CONCLUSION: LPS, TNFα, IL-6, dexamethasone and insulin significantly increase the expression of GPR54 in MCF7 cells, suggesting that these inflammatory factors and hormones may affect the function of gonadal axis by regulating the level of GPR54.