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①目的 探究青紫薯色素对60Coγ 射线辐射损伤小鼠的保护作用及机制。②方法 复制60 Coγ 射线(辐射强度为6 Gy)对雄性昆明种小鼠辐射损伤的病理模型。实验设计分为6组:对照组,60Co模型组,小剂量青紫薯色素组(青紫薯色素15 mg/kg),中剂量青紫薯色素组(青紫薯色素150 mg/kg),大剂量青紫薯色素组(青紫薯色素1 500 mg/kg)和尼尔雌醇阳性对照组(尼尔雌醇2 mg/kg),均灌胃给药,连续1个月。全血细胞分析仪计数小鼠外周血白细胞总数;计算小鼠的胸腺系数和脾脏系数;酶生化法测定小鼠脑、胸腺、肝、脾、肺组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH px)活性,活性氧(ROS)、丙二醛(MDA)含量以及总抗氧化能力(T AOC);油镜下检测小鼠骨髓细胞的微核率和染色体畸变率。③结果 在6 Gy 60Coγ 射线辐射情况下,青紫薯色素(灌胃剂量为15~1 500 mg/kg)能升高小鼠胸腺系数和脾脏系数,显著提高小鼠脑、胸腺、肝、脾、肺组织中SOD和 GSH px活性及T AOC,减少上述组织中ROS、MDA含量(F=14.07~1 315.68,q=5.51~80.49,P<0.01)。青紫薯色素的作用呈剂量依赖性。但青紫薯色素不能抑制辐射所致的小鼠白细胞总数下降、骨髓细胞产生微核及染色体畸变。④结论 青紫薯色素能抑制60Co辐射诱导的小鼠脑、胸腺、肝、脾、肺组织的
Objective To investigate the protective effect and mechanism of Qing purple potato pigment on 60Co γ-ray radiation injured mice. 2Methods The pathological model of radiation damage of male Kunming mice was replicated with 60 Coγ radiation (radiation intensity 6 Gy). The experimental design was divided into 6 groups: control group, 60Co model group, small dose of blue purple potato pigment group (blue purple potato pigment 15 mg/kg), middle dose blue purple potato pigment group (blue purple potato pigment 150 mg/kg), large dose of blue purple potato The pigment group (blue purple potato pigment 1 500 mg/kg) and nilestriol positive control group (nylestriol 2 mg/kg) were intragastrically administered for 1 month. The total number of white blood cells in peripheral blood of mice was counted by a whole blood cell analyzer; the thymus index and spleen coefficient of mice were calculated; and the superoxide dismutase (SOD) and glutathione in mouse brain, thymus, liver, spleen and lung tissues were measured by enzyme biochemical method. Peptide peroxidase (GSH px) activity, reactive oxygen species (ROS), malondialdehyde (MDA) content, and total antioxidant capacity (T AOC); micronucleus rate and chromosomal aberration rate of mouse bone marrow cells detected by oil microscopy . 3 Results In the case of 6 Gy 60Co γ-ray radiation, Qingyao Pigment (intragastric dose of 15~1 500 mg/kg) can increase the thymus index and spleen coefficient of mice, and significantly increase the brain, thymus, liver, spleen, The activities of SOD and GSH px and T AOC in lung tissue decreased the content of ROS and MDA in the above tissues (F=14.07~1 315.68, q=5.51~80.49, P<0.01). The effect of Qing purple potato pigment was dose-dependent. However, the purple purple potato pigment can not inhibit the decrease of the total number of white blood cells in mice caused by radiation, micronuclei of bone marrow cells and chromosomal aberrations. 4 Conclusions Purple potato pigment can inhibit the 60Co radiation induced mouse brain, thymus, liver, spleen, lung tissue