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目的:了解脂肪间充质干细胞(ASC)的培养上清的抗胶质瘤特性。方法:使用ASC培养上清培养胶质瘤细胞U251、U87,采用CCK-8检测ASC的培养上清抑制胶质瘤细胞U251、U87的增殖能力。利用流式细胞仪检测Annexin-Ⅴ的表达分析U251、U87在ASC上清诱导培养后的凋亡程度。划痕试验、Matrigel侵袭试验检测ASC的培养上清降低U251、U87侵袭的能力。结果:脂肪间充质干细胞能抑制胶质瘤U251、U87细胞的增殖,Annxin-V和PI的凋亡检测表明ASC-CM能诱导U251、U87细胞凋亡。划痕实验和Metrigel实验显示U251、U87细胞的迁移性降低和侵袭能力减弱。结论:脂肪间充质干细胞培养上清均能有效诱导胶质瘤U251、U87细胞的凋亡,并且均能降低胶质瘤细胞迁移及侵袭能力。我们的发现提示ASC培养上清有良好的抗肿瘤的特性,并可能用于未来的胶质瘤的治疗。
OBJECTIVE: To understand the anti-glioma properties of cultured supernatant of adipose-derived mesenchymal stem cells (ASC). Methods: The glioma cells U251 and U87 were cultured with ASC culture supernatant. The culture supernatant of ASC was detected by CCK-8 to inhibit the proliferation of glioma cells U251 and U87. Annexin-V expression by flow cytometry analysis U251, U87 in ASC supernatant induced apoptosis after culture. Scratch test, Matrigel invasion assay ASC supernatant to detect the ability of U251, U87 invasion. Results: Adipose-derived mesenchymal stem cells could inhibit the proliferation of glioma U251 and U87 cells. The detection of Annxin-V and PI showed that ASC-CM can induce the apoptosis of U251 and U87 cells. Scratch experiments and Metrigel experiments showed that the migration of U251 and U87 cells decreased and the invasion ability weakened. CONCLUSION: Adipose-derived mesenchymal stem cell supernatant can effectively induce the apoptosis of glioma U251 and U87 cells, and both can reduce the migration and invasion ability of glioma cells. Our findings suggest that ASC culture supernatants have good anti-tumor properties and may be used for the treatment of future gliomas.