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AIM: To investigate catalase(Kat A) and alkyl hydroperoxide reductase(Ahp C) antibodies of Helicobacter pylori as biomarkers for gastric cancer(GC).METHODS: This study included 232 cases and 264 controls. Recombinant Kat A and Ahp C proteins were constructed and the levels of antibodies were tested by indirect enzyme-linked immunosorbent assay(ELISA). Logistic regression was applied to analyze the relationships between Kat A, Ahp C and GC. The χ2 trend test was used to evaluate the dose-response relationships between serum Kat A and Ahp C antibody levels and GC. Receiver operating characteristic(ROC) curve was used to evaluate the screening accuracy of Kat A and Ahp C as biomarkers. Combined analysis was used to observe screening accuracy of predictors for GC.RESULTS: In all subjects, the association between Kat A and Ahp C and GC risk was significant(P < 0.001) with odds ratio(OR) = 12.84(95%CI: 7.79-21.15)and OR = 2.4(95%CI: 1.55-3.73), respectively. Kat A and Ahp C antibody levels were strongly related to GC risk with a dose-dependent effect(P for trend < 0.001). The area under the ROC(AUC) for Kat A was 0.806, providing a sensitivity of 66.81% and specificity of 86.36%; and the AUC for Ahp C was 0.615, with a sensitivity of 75.65% and specificity of 45.49%. The AUC was 0.906 for Kat A and flagella protein A(Fla A) combined analysis.CONCLUSION: Serum Kat A and Ahp C antibodies are associated with GC risk and Kat A may serve as a biomarker for GC. Kat A/Fla A combined analysis improved screening accuracy.
AIM: To investigate catalase (Kat A) and alkyl hydroperoxide reductase (Ahp C) antibodies of Helicobacter pylori as biomarkers for gastric cancer (GC) .METHODS: This study included 232 cases and 264 controls. Recombinant Kat A and Ahp C proteins were constructed and the levels of antibodies were tested by indirect enzyme-linked immunosorbent assay (ELISA). Logistic regression was applied to analyze the relationships between Kat A, Ahp C and GC. The χ2 trend test was used to evaluate the dose-response relationships between serum Kat A and Ahp C antibody levels and GC. Receiver operating characteristic (ROC) curve was used to evaluate the screening accuracy of Kat A and Ahp C as biomarkers. Combined analysis was used to observe screening accuracy of predictors for GC .RESULTS: In all subjects, the association between Kat A and Ahp C and GC risk was significant (P <0.001) with odds ratio (OR) = 12.84 (95% CI 7.79-21.15) and OR = 2.4 (95% CI 1.55-3.73) , respectively. Kat A and Ahp C antibody levels The area under the ROC (AUC) for Kat A was 0.806, providing a sensitivity of 66.81% and specificity of 86.36%; and the AUC for AhP C was 0.615 with a sensitivity of 75.65% and specificity of 45.49% The AUC was 0.906 for Kat A and flagella protein A (Fla A) combined analysis. CONCLUSION: Serum Kat A and Ahp C antibodies are associated with GC risk and Kat A may serve as a biomarker for GC. Kat A / Fla A combined analysis improved screening accuracy.