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使用不连续PAGE法,分析了10尾准噶尔雅罗鱼(Leuciscus merzbacheri)眼睛、鳃、皮、背部肌肉、鳍和肝胰脏6种组织的10种同工酶(LDH,CCO,EST,CAT,POD,ME,MDH,G6PD,GDH,ADH)的差异表达,并对部分同工酶基因位点及表达酶谱表型进行了分析,以期为其种质资源保护和开发以及遗传育种等方面的研究提供基础资料。结果显示,10种同工酶中9种在6种组织中出现了明显的组织差异性,仅CCO在6种组织中的差异性较小。在对准噶尔雅罗鱼的10种同工酶的遗传多样性分析中,共记录到了21个基因位点,其中Est-1、Me-B、s-MDH、G6pd-A、G6pd-B和Adh-A为多态性基因位点。多态位点比例为:P=6/21=28.57%。
The ten isozymes (LDH, CCO, EST, CAT, P <0.05) of six tissues in 10 eyes of Leuciscus merzbacheri, gill, POD, ME, MDH, G6PD, GDH and ADH), and analyzed some loci of isozymes and the phenotypes of their expressed enzymes, with a view to their conservation and development of germplasm resources and their genetic breeding Research provides the basic information. The results showed that 9 out of the 10 isozymes showed obvious tissue differences in 6 kinds of tissues, but only 6 differences in CCO. A total of 21 loci were recorded in the genetic diversity analysis of ten isoenzymes of Salvia miltiorrhiza: Est-1, Me-B, s-MDH, G6pd-A, G6pd-B and Adh -A is a polymorphic gene locus. The percentage of polymorphic loci was: P = 6/21 = 28.57%.