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目的通过建立胃癌裸鼠种植瘤模型观察维生素(Vit)K2联合苯那普利对人胃癌SGC-7901裸鼠种植瘤的影响,以探讨两者对胃癌细胞的作用及机制。方法通过人胃癌SGC-7901细胞接种建立胃癌裸鼠种植瘤模型后,随机分成对照组、Vit K2组、苯那普利组和联合组,每组10只,灌胃2周后处死;TUNEL法检测各组胃癌细胞凋亡情况,免疫组化法检测血管内皮生长因子(VEGF)、caspase 3的蛋白表达情况。结果与对照组相比,药物组能明显抑制瘤体生长,而联合组较单独药物组抑制作用更明显(P<0.05)。联合组在抑制胃癌细胞生长和诱导胃癌细胞凋亡方面的作用均优于单独药物组(P<0.05)。与对照组比,联合组的VEGF表达阳性率明显下降(P<0.05)。结论 Vit K2与苯那普利联合能抑制裸鼠胃癌细胞生长,两者联合有协同作用,其机制可能为通过诱导细胞凋亡及抑制VEGF的表达。
OBJECTIVE: To investigate the effect of vitamin K2 combined with benazapril on the growth of human gastric cancer SGC-7901 nude mice by establishing a model of gastric cancer xenografts in nude mice. Methods Human gastric cancer SGC-7901 cells were inoculated to establish the model of gastric cancer xenografts in nude mice and then randomly divided into control group, Vit K2 group, benazepril group and combination group, with 10 rats in each group. The apoptosis of gastric cancer cells in each group was detected. The protein expression of vascular endothelial growth factor (VEGF) and caspase 3 were detected by immunohistochemistry. Results Compared with the control group, the drug group obviously inhibited the growth of the tumor, while the combined group had more obvious inhibitory effect than the drug alone group (P <0.05). The combination group was better than single drug group in inhibiting the growth of gastric cancer cells and inducing the apoptosis of gastric cancer cells (P <0.05). Compared with the control group, the positive rate of VEGF expression in the combined group was significantly decreased (P <0.05). Conclusion The combination of VitK2 and benazepril inhibits the growth of gastric cancer cells in nude mice. The combination of VitK2 and benazepril may have a synergistic effect. The mechanism may be through inducing apoptosis and inhibiting the expression of VEGF.