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据报道,有可能应用同位素稀释技术筛选豆科植物具有固氮能力的品种或使用非豆科作物作为参照标准甄别根瘤菌系组合的效力。此种技术需要施用低剂量的~(15)N标记肥料,并运用Fried和Mellado推导的方程式,从下式中求出植株得自空气氮的比率a。
It has been reported that it is possible to use isotope dilution techniques to screen legumes for nitrogen-fixing varieties or to use non-leguminous crops as a reference standard to screen for combinations of rhizobial lines. This technique requires applying a low dose of ~ (15) N-labeled fertilizer and using the equation derived by Fried and Mellado to find the plant-derived air nitrogen a ratio from: