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本文采用β-内啡肽(β-EP)mRNA打点杂交,反相高效液相色谱和放免测定研究了冷适应SD雄性大鼠垂体、下丘脑、淋巴细胞(LC)和血浆β-EP及其mRNA的变化,结果为:(1)冷暴一周时垂体β-EP mRNA明显增加,从而激活细胞免疫功能。(2)免疫中枢下丘脑和LC β-EP mRNA在冷适应建立(冷暴二周)时增加。(3)血浆β-EP从冷暴二周起持续增加,增强细胞免疫功能。虽然LC和血浆β-EP产物增加,但是垂体β-EP mRNA量从冷暴二周起恢复到对照组水平,很可能是通过LC-垂体-下丘脑轴,信息反馈到中枢神经系统造成的。
In this paper, we studied the changes of pituitary, hypothalamus, lymphocyte (LC) and plasma β-EP in cold-adapted SD male rats using β-EP mRNA RBT, RP- HPLC and radioimmunoassay mRNA changes, the results were: (1) cold storm a week when the pituitary β-EP mRNA increased significantly, thereby activating cellular immune function. (2) Immunoregulatory hypothalamus and LC β-EP mRNA increased during cold acclimation (cold storm two weeks). (3) Plasma β-EP continued to increase from two weeks after cold storm to enhance cellular immune function. Although LC and plasma β-EP products increased, the amount of pituitary β-EP mRNA returned to control level from two weeks after cold storms, probably due to the feedback of LC-pituitary-hypothalamic axis to the central nervous system.