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本文以番茄品种“红吉星”子叶为对象 ,进行了离体培养再生体系建立研究。结果表明 ,番茄子叶离体培养过程中 ,影响不定芽发生的因素主要有 :培养基中细胞分裂素种类与浓度、培养基维生素组分、实生苗苗龄、暗培养时间等。试验结果表明 :番茄品种“红吉星”子叶不定芽发生率达 95 %以上的条件是 :实生苗苗龄 10 - 2 5天 ,BA浓度 1.0mg/L附加IAA0 .2mg/L ,子叶暗培养 10 - 13天。卡那霉素抗性水平为 75mg/L ,30 0mg/L以下的头孢霉素加入量对子叶分化无不良影响。
In this paper, the tomato variety “Hong Ji Xing” cotyledons as an object, carried out in vitro culture regeneration system established. The results showed that during the in vitro culture of tomato cotyledon, the main factors affecting the occurrence of adventitious buds were the types and concentrations of cytokinin in the medium, the vitamin composition in the medium, the seedling age of seedlings and the dark culture time. The results showed that the conditions of more than 95% adventitious buds of tomato variety “Hong Ji Xing” cotyledons were as follows: the seedling age was 10-25 days, IAA0.2mg / L with BA concentration 1.0mg / L, dark cotyledon culture 10 - 13 days. The level of kanamycin resistance of 75mg / L, 30 0mg / L of cefotaxime cotyledon differentiation had no adverse effect on differentiation.