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比较了2种低温预处理天数、诱导培养基中添加麦芽糖或蔗糖、添加较高或较低浓度生长素、愈伤组织转移时体积大小、分化培养基中添加不同浓度激素的大麦花培愈伤组织的诱导率、白苗和绿苗分化率。依据试验结果可以认为:25d左右的3～5℃大麦幼穗预处理;诱导培养基中添加3～4mg/L2,4-D、0.5～1.0mg/LNAA、80g/L麦芽糖;分化培养基中添加1.0～2.0mg/L6-BA、0.1～0.5mg/L NAA;愈伤组织体积为2～3mm时转入分化培养基等措施,有利于提高绿苗数量和减少白苗数量。
Two kinds of low temperature pretreatment days were compared, maltose or sucrose was added into the induction medium, auxin with higher or lower concentration was added, volume of callus was transferred, callus of barley was cultured with different concentration of hormone in differentiation medium Tissue induction rate, white seedlings and green plant differentiation rate. According to the test results, pretreatment of barley with 3 ~ 5 ℃ of about 25d can be considered; 3 ~ 4mg / L 2,4-D, 0.5 ~ 1.0mg / L NAA and 80g / L maltose were added into the induction medium; The addition of 1.0-2.0 mg / L 6-BA and 0.1-0.5 mg / L NAA, and the transformation of callus into differentiation media when the volume of the callus is 2-3 mm are favorable for increasing the number of green plantlets and reducing the number of white plantlets.