论文部分内容阅读
目的研究镍冶炼烟尘致DNA损伤作用。方法采用单细胞凝胶电泳(SCGE)技术,检测2份镍冶炼烟尘对小鼠NIH/3T3细胞的DNA损伤。结果相同染毒时间内,随着染毒剂量(除样品2中50.0μg/ml剂量染毒24h组)加大,细胞拖尾率增高,DNA断裂程度在4h达到高峰,分别为35.5%、69.7%、85.2%、41.3%、75.7%、89.2%。各受试物在相同剂量下,染毒4h时均较染毒2h和染毒24h细胞拖尾率高。NIH/3T3细胞受到镍冶炼烟尘的受试物作用后,在染毒2h,样品1、样品2各剂量组的细胞彗星尾DNA含量、尾长(除12.5μg/ml剂量组外)、尾动差(除样品1的12.5μg/ml剂量组外)均明显高于阴性对照组,差异有统计学意义(P<0.05)。在染毒4h,两样品对细胞DNA的损伤达到高峰,各剂量组的3个指标与对照组的差异有统计学意义(P<0.05)。染毒时间延长至24h,上述3个指标改变程度均较染毒4h减弱,样品1的彗尾长与对照组的差异无统计学意义(P>0.05)。结论镍冶炼烟尘可不同程度致NIH/3T3细胞DNA损伤。
Objective To study DNA damage caused by nickel smelting dust. Methods Single cell gel electrophoresis (SCGE) was used to detect the DNA damage of NIH / 3T3 cells in two nickel smelts. Results During the same exposure time, the rate of DNA trapping increased with the increase of exposure dose (except for the 50.0μg / ml dose of 24h in sample 2), and the DNA fragmentation peaked at 4h, which were 35.5% and 69.7 %, 85.2%, 41.3%, 75.7%, 89.2%. The test substance in the same dose, when exposed to 4h compared with 2h exposure and exposure to 24h high rate of cell tailing. NIH / 3T3 cells were exposed to the nickel smelt smoke for 2h, and the DNA content and tail length (except for the 12.5μg / ml dose group) of tail cells The difference (except 12.5μg / ml dose group in sample 1) was significantly higher than that in the negative control group (P <0.05). The DNA damage of the two samples peaked at 4h after exposure, and there was significant difference between the three indexes in each dose group and the control group (P <0.05). When the exposure time was extended to 24h, the change of the above three indexes was weaker than that after 4h exposure. There was no significant difference between the I-tail length of the sample 1 and the control group (P> 0.05). Conclusion Ni smelting dust can cause DNA damage in NIH / 3T3 cells to varying degrees.