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本文应用本实验室发现的着染HeLa细胞染色体鞘的一例红斑狼疮病人抗血清对分离出的蚕豆染色体及核和蚕豆根尖生长点压片进行了免疫荧光染色,从两种不同制片方法得到的染色体上均染出了清晰的染色体鞘免疫荧光。之后又用此血清对蚕豆三天龄幼苗中(去除子叶、种皮)提取出的总蛋白进行Western印迹分析,获得的带型显示分子量为41K,39K,18K,17K的蛋白质可与抗血清中的抗体特异性结合,另有分子量为87K,38K,35K,34K,32K的蛋白质也可能具有与抗体特异性结合的能力。
In this study, we found that the antisera of lupus erythematosus infected HeLa cell chromosome sheath found in our laboratory were used to immunofluorescein the chromosomes of the faba bean isolated from Vicia faba and the point-of-growth tablet of Vicia faba root tips from two different production methods Of the chromosomes were dyed clear chromosome sheath immunofluorescence. Then, the total protein extracted from the three-day-old seedling of Vicia faba (removal of cotyledons and seed coat) was further subjected to Western blot analysis using this serum. The obtained banding showed that the proteins with molecular weights of 41K, 39K, 18K and 17K were comparable to those of antiserum , And other proteins with molecular weights of 87K, 38K, 35K, 34K, 32K may also have the ability to specifically bind to the antibody.