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目的：探讨链脲佐菌素（STZ）树糖尿病（DM）动物模型的建立及其空腹血糖（FBG）标准和最佳药物剂量。方法：45只树随机分为正常对照组、STZ80、100、120、150mg/kg组5个组，测定其注药前、后FBG，并于注药后第4、10周处死，取胰腺做光镜、电镜检查。结果：FBG≥111mmol/L的树胰腺胰岛数量明显减少，胰岛直径也明显缩小，胰岛β细胞数量减少，肿胀，可见数量不等的β细胞脱颗粒，甚至无紫红色颗粒，淀粉染色阳性，符合DM成模动物胰腺病理改变。正常对照组、STZ 80mg/kg组无一成模；100mg/kg组患病率10%（1/10），仅维持1周即自然阴转；120、150mg/kg组患病率均为88.9%（8/9），前者维持时间短，注药后第4周自然阴转率50%（4/8），第10周为100%（8/8）；后者维持时间长，注药后第4周自然阴转率37.5%（3/8），第10周为62.5%（5/8）。150mg/kg组成模后第6周有1只树死亡，其余各组树 无一死亡。结论：STZ能诱导树FDBE-DM模型，树 DM模型标准为FBG≥11.1mmol/L，最佳STZ剂量为150mg/kg。
Objective: To investigate the establishment of animal model of streptozotocin (STZ) diabetes mellitus (DM) and its fasting blood glucose (FBG) standard and the best dosage. Methods: Forty-five trees were randomly divided into normal control group, STZ80, 100,120,150 mg / kg group, FBG was measured before and after injection, and were sacrificed at 4 and 10 weeks after injection Light microscopy, electron microscopy. Results: The amount of pancreatic islets in FBG≥111mmol / L decreased significantly, the diameter of pancreatic islets decreased significantly, the number of islet β cells decreased and swelling, and the number ofβcells with varying degrees of degranulation was visible, even without fuchsia granules. Starch staining was positive DM animal model of pancreatic pathology. The normal control group, STZ 80mg / kg group, a model; 100mg / kg group prevalence of 10% (1/10), maintaining only 1 week that natural Yin; 120,150mg / kg group were 88.9 % (8/9), the former maintained a short time, the first 4 weeks after injection of natural vaginal conversion rate of 50% (4/8), the first 10 weeks was 100% (8/8); the latter to maintain a long time, injection After 4 weeks of natural negative conversion rate of 37.5% (3/8), the first 10 weeks was 62.5% (5/8). At the 6th week after 150mg / kg die, one tree died, and none of the remaining trees died. Conclusion: STZ can induce tree FDBE-DM model. The standard of DM model is FBG≥11.1mmol / L, and the best STZ dose is 150mg / kg.