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采用微卫星技术分析辽宁锦州地区东北林蛙野生群体的遗传多样性,利用8对微卫星引物对林蛙群体37个个体进行PCR扩增,结果共检测到25个等位基因,大小在270～612bp之间,平均等位基因数量(NA)为3.375,平均观测杂合度(HO)为0.6356,平均期望杂合度(HE)为0.5846,平均多态信息含量(PIC)为0.5681,同时得到了两个杂合度较高的基因位点RsyD25和RsyD88。研究表明,该野生型群体的多态性良好,不存在退化现象。同时确定了杂合位点较多的个体,可以作为亲本在人工繁殖时使用。本研究丰富了野生型林蛙遗传多样性的数据,并为林蛙养殖过程中的选种提供了一定的理论依据。
Microsatellite DNA was used to analyze the genetic diversity of wild populations of Rana chensinensis in Jinzhou, Liaoning Province. By using 8 pairs of microsatellite primers to amplify 37 individuals of Rana chensinensis population, 25 alleles were detected, The average number of alleles (NA) was 3.375, the average observed heterozygosity (HO) was 0.6356, the average expected heterozygosity (HE) was 0.5846, the average polymorphism information content (PIC) was 0.5681, A higher heterozygous loci RsyD25 and RsyD88. Studies have shown that the wild-type population polymorphism is good, there is no degradation. At the same time, individuals with more heterozygous loci were identified, which can be used as artificial parents for propagation. This study enriched the data of genetic diversity of wild Rana chensinensis and provided some theoretical basis for the selection of Rana chensinensis breeding process.