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为了探讨金钗石斛提取物对慢性不可预见应激模型小鼠的抗抑郁作用,将BALB/c小鼠分为6组,即正常组、模型组、阳性药组(帕罗西汀),金钗石斛低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量组,灌胃给药两周后,除正常组外,其他组给予慢性不可预见性应激造模35天。造模结束后,通过糖水偏爱、新奇抑制摄食、强迫游泳实验和悬尾实验检测其行为学改变,采用LC-MS/MS测定各组小鼠应激后海马及皮层单胺类神经递质包括多巴胺(dopamine,DA)和5-羟色胺(5-hydroxytryptamine,5-HT)的含量改变。结果显示,与正常组相比,模型组小鼠糖水偏爱指数下降(P<0.01);与模型组比较,金钗石斛各剂量组可显著逆转模型小鼠出现的糖水偏爱指数下降(P<0.01),其作用与阳性药帕罗西汀相当;与正常组相比,模型组小鼠新奇抑制摄食潜伏期延长(P<0.01);与模型组比较,金钗石斛各剂量组均可显著缩短新奇抑制摄食潜伏期(P<0.01);与正常组相比,模型组小鼠悬尾的不动时间明显延长(P<0.01),与模型组比较,帕罗西汀及200 mg/kg金钗石斛均可缩短悬尾的不动时间(P<0.05);与正常组相比,模型小鼠在强迫游泳实验中不动时间无明显延长,各给药组的不动时间也未见明显改变(P>0.05)。与正常组相比,模型组小鼠皮层和海马中的DA和5-HT含量明显减少(P<0.05);帕罗西汀能使模型小鼠海马和皮层DA、海马5-HT含量明显增加(P<0.05);金钗石斛低、高剂量组皮层DA含量与模型组相比显著性升高(P<0.05),金钗石斛中、高剂量组海马DA含量与模型组相比显著性升高(P<0.05);金钗石斛高剂量组皮层和海马5-HT含量显著高于模型组(P<0.05),但金钗石斛中剂量组仅海马5-HT含量显著高于模型组(P<0.01)。以上结果提示,慢性不可预见应激可导致小鼠的类抑郁样行为出现,而金钗石斛提取物能有效改善慢性不可预见应激模型动物的抑郁样行为学表现,并提高小鼠脑内的DA和5-HT水平。
In order to investigate the antidepressant effect of Dendrobium nobile extract on chronic unpredictable stress model mice, BALB / c mice were divided into 6 groups: normal group, model group, positive group (Paroxetine), Dendrobium nobile (50 mg / kg), medium (100 mg / kg) and high (200 mg / kg) groups. After two weeks of intragastric administration, except for the normal group, other groups were given chronic unpredictable stress modeling 35 days. After modeling, behavioral changes were detected by using sugar-water preference, novel inhibition feeding, forced swimming test and tail-suspension test. Monoamine neurotransmitters in hippocampus and cortex were measured by LC-MS / MS. Dopamine (DA) and 5-hydroxytryptamine (5-HT) content changes. The results showed that, compared with the normal group, the model group mice syphilis preference index decreased (P <0.01); compared with the model group, each dose of Dendrobium nobile Lindsey group significantly reverse the decline in sugar-water preference index (P <0.01 ), Which was equivalent to the positive drug paroxetine. Compared with the normal group, the novelty of the model mice inhibited the latent period of feeding prolongation (P <0.01). Compared with the model group, each dose of Dendrobium nobile could significantly shorten the inhibition of food intake (P <0.01). Compared with the normal group, the immobility time of the tail suspension in the model group was significantly longer (P <0.01). Compared with the model group, paroxetine and 200 mg / kg Dendrobium nobile could shorten the suspension (P <0.05). Compared with the normal group, the model mice did not prolong the immobility time in the forced swimming test, and the immobility time of each group did not change significantly (P> 0.05) . Compared with the normal group, the content of DA and 5-HT in the cortex and hippocampus of the model group was significantly decreased (P <0.05); paroxetine significantly increased the content of DA and hippocampal 5-HT in the model mice (P <0.05). The content of DA in cortex of Dendrobium nobile Lindl with low and high dose groups was significantly higher than that of model group (P <0.05). The content of DA in hippocampus of high and low dose groups of Dendrobium nobile was significantly higher than that of model group (P <0.05). The content of 5-HT in the cortex and hippocampus of the high dose group of Dendrobium nobile was significantly higher than that of the model group (P <0.05), but the content of 5-HT in hippocampus of the Dendrobium nobile was significantly higher than that of the model group <0.01). The above results suggest that chronic unpredictable stress can lead to depression-like behavior in mice, and Dendrobium nobile extract can effectively improve the depressive-like behavior of chronic unpredictable stress model animals and improve the brain DA and 5-HT levels.