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用胶原酶灌注消化,收集小牛主动脉内皮细胞(EC)进行传代培养。培养的细胞具有典型的EC生长特征,呈单层镶嵌状生长,第Ⅷ因子间接荧光染色阳性。细胞传至20代生长稳定。
The collagenase digestion and digestion, collection of aortic endothelial cells (EC) were subcultured. The cultured cells have the typical EC growth characteristics, showing a monolayer mosaic growth, and the indirect staining of factor Ⅷ is positive. Cells spread to the 20th generation of stable growth.