论文部分内容阅读
目的观察环磷酰胺(CTX)节律化疗对Lewis肺癌肿瘤血管生成的影响。方法建立荷Lewis肺癌C57BL/6小鼠模型,随机分为CTX节律化疗组、CTX最大耐受剂量(MTD)组和对照组,于造模后第6天开始分别给予相应治疗。观察小鼠饮食、活动及一般状况,连续测量肿瘤大小和小鼠体质量。处死小鼠后,称瘤重,免疫组织化学方法测定各组肿瘤组织的微血管密度(MVD)及血管内皮生长因子(VEGF)表达情况。结果①与MTD组和对照组相比,节律化疗组肿瘤生长曲线较平缓,动物体质量无下降;②实验结束时,节律组、MTD组和对照组的平均瘤重分别为(1.68±0.37)g、(1.94±0.56)g、(3.75±0.86)g;③节律组、MTD组和对照组的MVD分别为15.09±3.03、23.51±2.78和26.38±2.56,VEGF高表达率分别为46.2%、73.5%和81.3%。结论CTX节律化疗可显著抑制Lewis肺癌的生长及其血管生成,且毒副作用较MTD化疗小。
Objective To observe the effects of cyclophosphamide (CTX) rhythm chemotherapy on angiogenesis in Lewis lung carcinoma. Methods The Lewis lung carcinoma C57BL / 6 mouse model was established and randomly divided into CTX rhythm chemotherapy group, CTX MTD group and control group, and the corresponding treatment was given on the 6th day after the model was established. Observe the mice diet, activity and general condition, continuous measurement of tumor size and body weight of mice. After the mice were sacrificed, the tumor weight and the expression of vascular endothelial growth factor (VEGF) were measured by immunohistochemistry. Results ① Compared with the MTD group and the control group, the tumor growth curve of the rhythm chemotherapy group was gentle and the body weight of the animals did not decrease. ② At the end of the experiment, the mean tumor weights of the rhythm group, the MTD group and the control group were (1.68 ± 0.37) (1.94 ± 0.56) g and (3.75 ± 0.86) g, respectively; ③ The MVD of rhythm group, MTD group and control group were 15.09 ± 3.03,23.51 ± 2.78 and 26.38 ± 2.56 respectively, the high expression rates of VEGF were 46.2% 73.5% and 81.3%. Conclusion CTX rhythm chemotherapy can significantly inhibit the growth and angiogenesis of Lewis lung carcinoma with less side effects than MTD chemotherapy.