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本实验分别将日本血吸虫(大陆株)雌、雄、合抱虫体可溶性抗原制剂,佐以卡介苗,一次性皮内免疫小鼠。结果,免疫后的小鼠对血吸虫的攻击产生相当的保护作用(保护率分别为42.3％、42.6％和44.5％)。经SDS-PAGE和Western Blot对虫体可溶性抗原进行分析,免疫血清为1:300时,仅有一条蛋白带出现强而明显的反应,其反应蛋白带的分子量为94/90kD。
In this study, we separately immunized mice with Schistosoma japonicum (Continent strain) female, male and female with soluble antigens against Bacillus Calmette-Guerin (BCG) once intradermally. As a result, the immunized mice gave considerable protection against schistosomiasis (protection rates of 42.3%, 42.6% and 44.5%, respectively). Soluble antigens were analyzed by SDS-PAGE and Western Blot. When the immune serum was 1: 300, only one protein band appeared strong and obvious reaction, and the molecular weight of the reaction protein band was 94/90 kD.