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背景:甜菜碱可改善酒精性肝病和非酒精性脂肪性肝病患者的血清生化指标和肝组织学变化,但其机制尚不明确。目的:研究甜菜碱对鹌鹑高脂饮食性脂肪肝的影响。方法:制备鹌鹑高脂饮食性脂肪肝动物模型,以甜菜碱干预后,观察其血清、肝脂质、肝功能生化指标和脂质过氧化指标的变化,并观察肝组织学改变。结果:予高脂饮食性脂肪肝鹌鹑甜菜碱0.2～0.8g/kg治疗6周后,其血清总胆固醇、三酰甘油、低密度脂蛋白-胆固醇、游离脂肪酸水平、肝重指数、肝组织总胆固醇均显著降低(P<0.05或P<0.01);肝组织超氧化物歧化酶活性升高(P<0.01);0.8g/kg甜菜碱组可升高血清高密度脂蛋白-胆固醇水平(P<0.05),降低肝组织三酰甘油和丙二醛含量(P<0.01)。组织病理学检查结果显示,甜菜碱可使肝细胞脂肪变性程度明显减轻(P<0.05或P<0.01),0.8g/kg组的肝细胞结构趋于正常。结论:甜菜碱对鹌鹑高脂饮食性脂肪肝有较好的治疗作用,其部分机制可能是通过抗脂质过氧化和增加脂质代谢实现的。
BACKGROUND: Betaine improves serum biochemical and hepatic histological changes in patients with alcoholic liver disease and non-alcoholic fatty liver disease, but the mechanism is still unclear. Objective: To study the effect of betaine on fatty liver of quail high fat diet. Methods: The animal model of fatty liver of quail with high-fat diet was prepared. The serum, hepatic lipid, hepatic biochemical indexes and lipid peroxidation indexes were observed after the intervention of betaine, and the changes of liver histology were observed. Results: The serum levels of total cholesterol, triglyceride, low density lipoprotein - cholesterol, free fatty acid, liver mass index, total liver tissue, (P <0.05 or P <0.01), and the activities of superoxide dismutase (SOD) in liver tissue increased (P <0.01). The levels of serum high density lipoprotein-cholesterol <0.05), reduce the content of triglyceride and malondialdehyde in liver tissue (P <0.01). Histopathological examination showed that betaine could significantly reduce the degree of hepatic steatosis (P <0.05 or P <0.01), and the structure of hepatocytes in 0.8g / kg group tended to be normal. Conclusion: Betaine has a good therapeutic effect on fatty liver of quail high-fat diet. Part of the mechanism may be through lipid peroxidation and lipid metabolism.