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大豆异黄酮具有特定的生理活性,因此引起人们的广泛关注。研究大豆籽粒发育过程中异黄酮含量及其合成底物含量的变化规律,分析造成大豆籽粒异黄酮含量差异的合成代谢底物方面的原因,对于利用传统或分子生物学手段改良大豆籽粒异黄酮含量均具有重要意义。为此,选用大豆籽粒异黄酮含量差异较大的6份材料统一种植,在籽粒发育的R5、R6、R7和R8期分别取籽粒提取测定异黄酮、游离苯丙氨酸、香豆酸和柚皮素含量。结果表明:大豆异黄酮的3种苷元总含量及大豆黄素、染料木素含量均随生育进程逐渐升高;大豆黄素先于染料木素在大豆种子中开始积累;大豆籽粒中游离苯丙氨酸和柚皮素含量随种子发育进程含量逐渐降低,香豆酸含量逐渐升高;不同品种异黄酮含量在各生育阶段与游离苯丙氨酸含量多呈负相关,与香豆酸含量多呈正相关。
Soy isoflavones have specific physiological activity and therefore attract people’s attention. To study the variation rules of isoflavone content and its synthetic substrate content in the process of soybean seed development, and to analyze the reasons of the anabolic substrates that cause the differences in the content of isoflavones in soybean seeds, it is of great significance to improve the isoflavone content of soybean seeds by traditional or molecular biological methods All of great significance. Therefore, we selected six materials with different contents of isoflavone in soybean seeds to be planted in a uniform manner. Grain extracts were used to determine isoflavone, free phenylalanine, coumaric acid and grapefruit in the stages R5, R6, R7 and R8 of grain development Skin content. The results showed that the total content of three aglycones and the content of daidzein and genistein in the soybean isoflavones increased gradually with the growth process. The daidzein began to accumulate in the soybean seeds before genistein began to accumulate, The content of acid and naringenin gradually decreased with the development of seeds and the content of coumaric acid gradually increased. The isoflavone content of different varieties had mostly negative correlation with free phenylalanine content at each growth stage, Related.