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目的:研究褐藻匍扇藻(Lobophora variegata)多糖的抗病毒活性。方法:匍扇藻多糖的粗提物经沸水提取和乙醇沉淀后获得,粗多糖经离子交换柱层析法分离以获取较纯的多糖片段。它们的抗病毒活性采用细胞病变效应减少法和空斑减少检测法检测。粗多糖的细胞毒性采用 MTT 法测定。结果:匍扇藻粗多糖具有显著的抗单纯疱疹病毒Ⅰ型和Ⅱ型的活性,它能抑制不同的单纯疱疹病毒毒株,包括标准株、阿昔洛韦抗性株和临床病毒株。通过空斑减少法测得它对阿昔洛韦抗性株和 HSV-Ⅱ型临床株的半数有效浓度(EC50)分别为 18.2 和 6.25 μg/ml。因为它的细胞毒性很低,因此具有较大的选择性系数。这种粗多糖有一定的抗呼吸道合胞病毒(RSV)活性,但对流感病毒没有抑制作用。该粗多糖经DEAE离子交换柱层析分离得到两个不同的多糖,它们也都显示出抗单纯疱疹病毒的活性。结论:匍扇藻含有具抗病毒活性的多糖并且对单纯疱疹病毒显示出较强的抑制作用。
Objective: To study the antiviral activity of Lobophora variegata polysaccharides. Methods: The crude extracts of polysaccharides of S. sibirica were obtained by boiling water extraction and ethanol precipitation. The crude polysaccharides were separated by ion-exchange chromatography to obtain relatively pure polysaccharide fragments. Their antiviral activity was measured using cytopathic effect reduction assays and plaque reduction assays. The cytotoxicity of the crude polysaccharide was measured by the MTT method. RESULTS: The crude polysaccharides of S. elegans had significant anti-herpes simplex virus type I and type II activity, and it inhibited different herpes simplex virus strains, including standard strains, acyclovir-resistant strains, and clinical virus strains. The median effective concentration (EC50) of acyclovir-resistant and HSV-II clinical isolates was 18.2 and 6.25 μg/ml, respectively, determined by plaque reduction. Because of its low cytotoxicity, it has a large selectivity factor. This crude polysaccharide has a certain anti-respiratory syncytial virus (RSV) activity but no inhibitory effect on influenza virus. The crude polysaccharide was separated by DEAE ion exchange column chromatography to obtain two different polysaccharides, which also showed activity against herpes simplex virus. Conclusion: The algae contains antiviral activity polysaccharides and shows a strong inhibitory effect on herpes simplex virus.