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目的 探讨c-IAPl蛋白和mRNA在胃腺癌组织中的表达及临床意义.方法 采用免疫组化SP法、Western blot和qRT-PCR技术检测50例胃腺癌组织和40例癌旁正常胃黏膜组织中c-IAPl蛋白和mRNA的表达,分析其在胃腺癌发生、发展中的作用及与临床病理特征的关系.结果 c-IAPl蛋白和mRNA在胃腺癌组织中的表达水平明显高于癌旁正常胃黏膜组织(P均<0.05).胃腺癌组织中c-IAPl蛋白和mRNA的表达与组织分化程度、TNM分期、淋巴结转移、浸润深度均相关(P＜0.05),与患者性别、年龄无关(P＜0.05).结论 c-IAPl在胃腺癌组织中表达升高,抑制了胃腺癌细胞的凋亡,促进了胃腺癌的发生、发展,有可能为胃腺癌的临床靶向治疗提供新的理论依据.“,”Purpose To investigate the protein and mRNA expression of c-IAPl in gastric adenocarcinoma tissue and its clinical significance. Methods Immunohistochemistry technique, Western blot and realtime fluorescent quantitative PCR(qRT-PCR) were used to detect the protein and mRNA expression of c-IAPl in 50 cases of gastric adenocarcinoma tissues and40 cases of adjacent normal gastric mucosa tissues to analyze its role in the development and progression of gastric adenocarcinoma, the relationship between the protein and mRNA expression of c-IAPl and the clinicopathological features. Results The relative expression level of c-IAPl protein in gastric adenocarcinoma tissues was significantly higher than that in adjacent normal gastric mucosa tissues, the difference was statistically significant (P＜ 0.05 ). The mRNA expression of c-IAPl in gastric adenocarcinoma was significantly higher than normal gastric mucosa tissues, the difference was statistically significant (P＜0.05). The protein and mRNA expression of c-IAPl were correlated with the degree of differentiation of gastric adenocarcinoma tissues, TNM clinical stage, lymph node metastasis and infiltration depth, the difference was statistically significant (P＜0.05 ), while there was no correlation with gender and age, the difference was not statistically significant (P＞ 0.05). Conclusion The high protein and mRNA expression of c-IAPl in gastric adenocarcinoma tissues inhibit the apoptosis of gastric adenocarcinoma cells, which contribute to the development and progression of gastric carcinoma and it may provide a new theoretical basis for the clinical targeted therapy of gastric adenocarcinoma.