论文部分内容阅读
目的通过分析mtDNA拷贝量在乳腺癌组织和癌旁正常组织中的变化,探讨其在乳腺癌发生和发展中的作用。方法采用PCR反应分别扩增20例乳腺浸润性导管癌组织和相对应的20例癌旁正常组织的mtDNAD-loop两个高突变区HV1和HV2,并以核基因组-βactin作为定量标准物,经过聚丙烯酰胺凝胶电泳法(PAGE)并利用Gel-Pro Express图像分析系统进行核酸定量分析,比较mtDNA拷贝量在癌组织和癌旁组织间的差异。结果在乳腺癌组织中HV1和HV2的条带的吸光度均较癌旁正常组织弱,其中HV1在两者比较中有统计学差异(P<0.05);HV2在两者比较中有明显统计学差异(P<0.01);统计学分析发现,HV1和HV2呈明显正相关(P<0.01),且mtDNA的拷贝量与年龄、肿瘤大小均无统计学意义,而与淋巴结转移和肿瘤分期有统计学意义(均为P<0.05)。结论乳腺浸润性导管癌组织mtDNA拷贝量减少,与乳腺癌的发生和发展有密切关系,并可能成为一种新的乳腺癌标志物。
Objective To investigate the role of mtDNA copy number in the occurrence and development of breast cancer by analyzing the changes of mtDNA copy number in breast cancer tissues and adjacent normal tissues. Methods The HV1 and HV2 of mtDNAD-loop were amplified by PCR from 20 cases of invasive ductal carcinoma of breast and corresponding normal tissues of 20 adjacent normal tissues. The nuclear genomic-β actin was used as a quantitative standard, Polyacrylamide gel electrophoresis (PAGE) and quantitative analysis of nucleic acid using Gel-Pro Express image analysis system to compare mtDNA copy number differences in cancer tissue and adjacent tissues. Results The absorbance of HV1 and HV2 bands in breast cancer tissues were weaker than those in adjacent normal tissues, with HV1 statistically significant difference (P <0.05). There was significant difference between HV2 and HV2 (P <0.01). Statistical analysis showed that there was a significant positive correlation between HV1 and HV2 (P <0.01), and there were no significant differences in mtDNA copy number, age and tumor size between lymph node metastasis and tumor stage Significance (all P <0.05). Conclusion The decrease of mtDNA copy number in breast invasive ductal carcinoma is closely related to the occurrence and development of breast cancer and may be a new marker of breast cancer.