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目的探讨E-选择素基因SER128ARG(S128R)多态性在急性心肌梗死(AMI)患者和正常人群中的分布特点及与AMI的易感相关性,以及对血清E-选择素水平的影响。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,检测168例AMI患者和200例健康对照者E-选择素基因多态性,同时采用酶联免疫吸附试验(ELLSA)法检测所有参与者血清E-选择素水平。结果E-选择素基因S128R基因型频率和等位基因频率,在AMI组和对照组的分布差异有统计学意义(P<0.05);基因型频率的相对风险分析发现,SR杂合子基因型携带者患AMI的风险是SS纯合子基因型的2.234倍(OR=2.234,95%CI:1.112～4.437);AMI组血清E-选择素水平明显高于对照组(P<0.05);SR杂合子基因型携带者血清E-选择素水平显著高于SS纯合子基因型(41.65±8.87)μg/L和(34.23±6.72)μg/L,P<0.05。结论E-选择素S128R基因多态性与AMI的发病有关联,并影响血清E-选择素水平;提示R基因可能是AMI发病的遗传易感基因。
Objective To investigate the distribution of E-selectin gene SER128ARG (S128R) polymorphism in patients with acute myocardial infarction (AMI) and its normal population and its association with susceptibility to AMI and its effect on serum E-selectin levels. Methods The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the E-selectin gene polymorphism in 168 patients with AMI and 200 healthy controls. ELISA was used to detect E-selectin ) Method was used to detect serum E-selectin levels in all participants. Results The frequency of S128R genotype and allele frequency of E-selectin gene were significantly different between AMI group and control group (P <0.05). The relative risk of genotype frequency found that SR heterozygous genotype The risk of AMI was 2.234 times that of SS homozygote (OR = 2.234,95% CI: 1.112 ~ 4.437). The serum E-selectin level in AMI group was significantly higher than that in control group (P <0.05) Serum E-selectin levels of genotype carriers were significantly higher than SS homozygote (41.65 ± 8.87) μg / L and (34.23 ± 6.72) μg / L, P <0.05. Conclusion The E-selectin S128R gene polymorphism is associated with the pathogenesis of AMI and affects the serum E-selectin level. It suggests that R gene may be the genetic predisposition to AMI.