论文部分内容阅读
目的: 探讨人细小病毒B19感染与小儿川崎病的关系.方法: 用巢式PCR法对46例川崎病患儿进行B19 DNA检测,用ELISA法对其中的30例患儿进行B19 VP2 IgM检测.结果: 病例组46例,B19 DNA阳性14例( 30% ),对照组50例B19 DNA阳性2例,两组间差异显著(P<0 01).病例组B19 VP2 IgM阳性7 /30例,对照组50例均阴性.两组间差异极显著(P<0 01). 30例川崎病患者中B19 DNA,B19 VP2 IgM均阳性6例; 1例仅B19 VP2 IgM阳性; 4例仅B19 DNA阳性;B19 DNA和B19 VP2 IgM同时阴性19例,B19 DNA和B19 VP2 IgM一致率为83 3%,有一致性(P<0 01 ).B19阳性与阴性川崎病患儿在性别、年龄、常见临床表现、预后等方面无差别.结论: 我国川崎病患儿B19病毒感染率较高可能是导致川崎病的主要病原体之一.
Objective: To investigate the relationship between human parvovirus B19 infection and childhood Kawasaki disease.Methods: B19 DNA was detected in 46 children with Kawasaki disease by nested PCR method, and 30 cases of children with B19 VP2 IgM were detected by ELISA. Results: There were 46 cases in the case group and 14 cases (30%) in the B19 DNA positive group, and 50 cases in the control group were positive for B19 DNA in 2 cases (P <0.01) .The positive rate of B19 VP2 IgM was 7/30 in the case group, The control group of 50 patients were negative, the difference was significant between the two groups (P <0.01) .Among the 30 Kawasaki disease patients, B19 DNA and B19 VP2 IgM were all positive in 6 cases, only B19 VP2 IgM was positive in 1 case, and only B19 DNA was found in 4 cases 19 cases with B19 DNA and B19 VP2 IgM were negative at the same time, the concordance rate between B19 DNA and B19 VP2 IgM was 83.3% (P <0.01) .B19 positive and negative children with Kawasaki disease had significant difference in sex, age, Clinical manifestations, prognosis, etc. Conclusion: The high prevalence of B19 virus in children with Kawasaki disease may be one of the major pathogens causing Kawasaki disease.