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目的:观察青芪散对免疫损伤性小鼠自然杀伤细胞(NK细胞)和白细胞介素-2(IL-2)的影响,并探讨其机制。方法:将72只小鼠随机分为6组,每组12只,除正常对照组外,其余5组小鼠均采用卡介苗+脂多糖造模。于造模第二天起青芪散小、中、大剂量组小鼠以青芪散小、中、大3个剂量灌胃;迈普新组小鼠以迈普新腹腔注射;病理组和正常对照组小鼠以氯化钠溶液灌胃。实验共10天,处死后观察各组小鼠NK细胞、IL-2及肝脏病理情况。结果:青芪散中剂量组小鼠NK细胞、IL-2水平显著增高,肝组织病理学改变与病理组、青芪散小剂量组比较,差异有显著性意义(P<0.05或P<0.01)。与迈普新组比较,差异无显著性意义。结论:青芪散对免疫损伤性小鼠非特异性免疫机制和细胞因子的网络调控有调节作用。
OBJECTIVE: To observe the effect of Qingqi powder on natural killer cells (NK cells) and interleukin-2 (IL-2) in mice with immune injury and to explore its mechanism. Methods: 72 mice were randomly divided into 6 groups, 12 in each group. Except the normal control group, the other 5 groups were all made of BCG + lipopolysaccharide. On the second day after model establishment, the mice in the small, medium and high dose group of Qingdai San received intragastric administration of small intestine, medium and large doses of Qinglan San. The mice in the Maipuxin group were injected intraperitoneally with Maipuxin; Normal control mice were intragastrically administered sodium chloride solution. After a total of 10 days in the experiment, the NK cells, IL-2, and liver pathological changes were observed in each group after sacrifice. RESULTS: The NK cells and IL-2 levels in the middle-dose group of Qingqi powder were significantly increased, and the histopathological changes in the liver were significantly different from those in the pathological group and the Qingdaisan low-dose group (P<0.05 or P<0.01). ). Compared with the Maipuxin group, the difference was not significant. Conclusion: Qingqi Powder can regulate the non-specific immune mechanism and network regulation of cytokines in immunocompromised mice.