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目的分析艾滋病病毒Ⅰ型(HIV-1)确证实验中不确定结果的免疫印迹带型,为诊断新发感染提供实验室数据。方法酶联免疫吸附试验(ELISA)与免疫印迹试验(WB)按照试剂说明书及《全国艾滋病检测技术规范》(2004年版)进行,HIV-1病毒载量检测使用罗氏公司的HIV-1 1.5 version试剂盒。结果在感染的早期,血清中的抗体含量较低,ELISA检测时的OD值仅为CUTOFF值的2.5至3倍,但在感染2-3个月后,抗体水平显著升高。在第2次检测中OD值升高,WB条带颜色加深,条带数目增加。结论为了避免二代传播的发生和早期感染者的流失,应适当缩短随访复检的时间。
OBJECTIVE: To analyze the immunoblot patterns of indeterminate results in HIV-1 confirmatory experiments and provide laboratory data for the diagnosis of new infections. Methods Enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) were performed according to the reagent manual and the National AIDS Testing Technical Specification (2004 Edition). The HIV-1 viral load was detected using Roche HIV-1 1.5 version reagent box. Results In the early stages of infection, serum antibody levels were low, with OD values of 2.5 to 3 fold higher than the CUTOFF values detected by ELISA, but significantly increased after 2-3 months of infection. In the second test, the OD value increased, the WB band color deepened, and the number of bands increased. Conclusion In order to avoid the second-generation transmission and early infection loss, the time of follow-up review should be appropriately shortened.