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在胡椒(PipernigrumLinn.)茎尖丛生增殖技术的基础上,以印尼大叶种“LampongType”无菌实生苗作外植体源,利用辣椒疫霉(Phytophthoracapsici)培养滤液对胡椒茎尖及其增殖形成的丛生芽进行体外选择。辣椒疫霉培养滤液的不同灭菌方法对辣椒疫霉培养滤液的毒性影响显著,过滤灭菌方式可以保持辣椒疫霉培养滤液的毒性,而高温高压灭菌方式则不能。随着辣椒疫霉培养滤液浓度的增加,茎尖和丛生芽的存活率和增殖率都在下降。在存活的茎尖或丛生芽培养中,一部分可正常增殖,其余的形成愈伤组织,或者保持生长停滞的休眠状态。在选择性培养基上继代培养2次后进行生根和移栽,利用离体叶片针刺接种法对温室条件下生长的移栽植株进行抗瘟病测定。以3次抗病检测均无明显症状的植株作为抗病株。随着辣椒疫霉培养滤液浓度的增加,得到的再生植株数量降低,但其中抗病株的比例提高。利用过滤灭菌方式加入选择性培养基的处理中,25%、50%和75%的辣椒疫霉培养滤液分别获得1株、4株和3株抗病株,分别占各处理再生植株总数的1.54%、20.00%和42.86%,共获得8株,占该组处理再生植株总数的8.70%。
Based on the shoot tip proliferation of Pepper (Piper nigrum Linn.), The sterile shoot of “Big Seed” Lampong Type was used as explants and the filtrate of Phytophthora capsici was used to culture the shoot tips and their proliferation In vitro selection of clusters of buds. Different sterilization methods of Phytophthora capsici culture filtrate have significant effects on the toxicity of Phytophthora capsici culture filtrate. The filtration sterilization method can maintain the phytotoxicity of Phytophthora capsici culture filtrate, but the autoclave sterilization method can not. As the concentration of Phytophthora capsici culture filtrate increased, the survival rate and proliferation rate of shoot tips and shoots decreased. In surviving shoot tip or shoot culture, a part of the normal proliferation, the formation of callus or the rest of the growth to maintain a dormant state. After subculture on selective medium for 2 times, the rooting and transplanting were carried out. The leaf blight inoculation method was used to test the resistance of the transplanted plants grown under greenhouse conditions. The three disease-free tests showed no obvious symptoms of plants as disease-resistant strains. As the concentration of Phytophthora capsici culture filtrate increased, the number of regenerated plants decreased but the proportion of resistant plants increased. In the treatment of adding selective medium by filter sterilization, 25%, 50% and 75% of Phytophthora capsici culture filtrates obtained 1, 4 and 3 resistant strains, respectively, accounting for the total number of regenerated plants 1.54%, 20.00% and 42.86% respectively. A total of 8 strains were obtained, accounting for 8.70% of the total number of regenerated plants in this group.