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考察不同浓度(50,100,200μg/mL)黄原胶(XG)对脂多糖(LPS)刺激的兔膝骨关节软骨细胞氧化损伤的影响。玻璃酸钠(SH)、维生素C(Vc)作为阳性对照药,采用流式细胞术和荧光酶标仪检测活性氧(ROS)表达,试剂盒检测一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)的含量。两种ROS检测方法显示,与模型组相比,XG各剂量组均能降低ROS水平,XG与SH无显著性差异,Vc的抗氧化能力强于XG及SH。与模型组相比,XG、SH均能减少LPS诱导软骨细胞产生的NO,SH较为显著。XG、SH能显著降低LPS诱导产生的MDA,高剂量组XG优于SH。XG能增强LPS诱导兔软骨细胞产生SOD的活性。综上所述,XG能逆转LPS造成的兔软骨细胞氧化损伤,消除ROS,提高SOD活性,降低MDA、NO水平,是OA的潜在治疗药物。
To investigate the effects of XG (50, 100, 200μg / mL) xanthan gum on oxidative damage of rabbit knee joint articular cartilage cells stimulated by lipopolysaccharide (LPS). Sodium hyaluronate (SH) and vitamin C (Vc) were used as positive control drugs. The expression of reactive oxygen species (ROS) was detected by flow cytometry and fluorescent microplate reader. Nitric oxide (NO), malondialdehyde (MDA) ), Superoxide dismutase (SOD) content. The results of two ROS assays showed that compared with the model group, each dose of XG could reduce the level of ROS. There was no significant difference between XG and SH. The antioxidant capacity of Vc was stronger than that of XG and SH. Compared with the model group, XG and SH can reduce the LPS-induced chondrocytes NO, SH is more significant. XG, SH can significantly reduce the LPS-induced MDA, high-dose group XG better than SH. XG can enhance the LPS-induced activity of rabbit chondrocytes producing SOD. In conclusion, XG can reverse the oxidative damage of rabbit chondrocytes induced by LPS, eliminate ROS, increase the activity of SOD and decrease the level of MDA and NO. It is a potential therapeutic drug for OA.