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目的 观察蝎毒抗癌多肽 (APBMV)与放疗 (RT)合用对肝癌小鼠的作用。方法 取H2 2 带瘤小鼠 10 0只 ,采用肿瘤生长抑制率 (IR)、白细胞计数和血红蛋白含量、SOD活力、LPO含量测定及脾重指数 (SI)等指标 ,观察不同剂量的APBMV与放疗合用后上述指标的变化。结果 放疗后 6d及 9d ,RT与APBMV合用后瘤重明显降低 ,IR最高达 78 2 9%与 70 45 % (与RT组和APB MV组比较 ,P <0 0 5 ,或P <0 0 1) ,白细胞计数和SI较RT组显著升高 (P <0 0 5 ,P <0 0 1) ;血红蛋白含量测定结果无明显差异 ;RT组SOD活力最低 ,LPO含量最高 (与对照组比较P <0 0 5 ,P <0 0 1) ,RT +APBMV高剂量组SOD活力明显提高 ,LPO含量显著降低 (P <0 0 1) ,接近空白对照水平。结论 APBMV与放疗合用对H2 2 的抑制作用比单纯放疗和单用APBMV增强 ,APBMV并能减轻辐射对带瘤小鼠机体的损伤。
Objective To observe the effect of combination of APBMV and radiotherapy (RT) on HCC mice. Methods A total of 100 H2 2 tumor-bearing mice were used. The tumor growth inhibitory rate (IR), white blood cell count and hemoglobin content, SOD activity, LPO content and spleen weight index (SI) were used to observe different doses of APBMV and radiotherapy. Changes in the above indicators after combined use. Results On the 6th and 9th days after radiotherapy, the tumor weight was significantly decreased after combined RT and APBMV treatment. The highest IR was 78 2 9% and 70 45 % (compared with RT group and APB MV group, P <0 05, or P <0 01. The white blood cell count and SI were significantly higher than those in the RT group (P < 0.05, P <0 01). There was no significant difference in the hemoglobin content. The RT group had the lowest SOD activity and the highest LPO content (compared with the control group P < 0 0 5, P <0 0 1), SOD activity in high dose RT + APBMV group was significantly increased, LPO content was significantly decreased (P <0 01), close to the blank control level. Conclusion APBMV combined with radiotherapy has stronger inhibitory effect on H2 2 than radiotherapy alone and APBMV alone. APBMV can reduce the radiation damage to tumor-bearing mice.