硫酸糖肽作为一种细胞保护因子,能显著抑制胃蛋白酶活性,适用于治疗胃、十二指肠溃疡和胃炎。文章在氯磺酸-吡啶法硫酸酯化多糖的基础上,通过优化酯化条件,建立了硫酸糖肽制备方法及检测方法,实验以肠多糖为原料,采用氯磺酸与吡啶(体积比为1∶5)反应制备酯化试剂,在70℃下,酯化反应5 h,再通过有机溶媒和透析等提纯方法制得硫酸糖肽,产品经电泳鉴定分析、硫含量等指标测定,均达到现有的硫酸糖肽标准品的质量指标。 Glycopeptide sulfate as a cytoprotective factor, can significantly inhibit pepsin activity, is suitable for the treatment of stomach, duodenal ulcer and gastritis. Based on the chlorosulfonic acid-pyridine sulfated polysaccharide, the preparation method and the detection method of glycopeptide sulfate were established by optimizing the esterification conditions. The experiment was carried out by using polysaccharide as raw material, chlorosulfonic acid and pyridine 1: 5), esterification reaction was carried out at 70 ℃ for 5 h, and then the glycopeptide was obtained by purification by organic solvent and dialysis. The product was identified by electrophoresis, sulfur content and other indicators were reached Existing quality standards of sulfated glycopeptide standards.