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本试验选用6个抗病性不同的甘蓝型油菜品种,研究其叶表皮蜡质组成及结构与菌核病抗性的关系。结果表明,抗病品种在去除叶表皮蜡质后病情指数显著增加;感病品种无显著变化。不同抗性品种(系)间除酯类组分含量无显著差异外,其余蜡质组分含量差异显著。相关分析表明,蜡质组分中酯类含量与病情指数呈显著负相关关系,醇类、酮类含量与病情指数呈显著正相关,其余组分和蜡质总量与病情指数无显著相关关系。抗性品种叶表皮蜡质中烷类及酯类所占比重较高,而易感品种酮类比重较高。扫描电镜结果显示,抗病品种(系)的蜡质晶体主要为颗粒状、杆状、丝状;而感病品种(系)的蜡质晶体中不规则片状晶体所占比例较大。这些结果说明油菜叶表皮蜡质的组分及结构可能是抗病品种抵抗和延迟病原菌侵入的机制之一。
In this study, six different rape varieties were used to study the relationship between the wax composition and structure of leaf epidermis and Sclerotinia sclerotiorum resistance. The results showed that the disease index of resistant varieties increased significantly after the removal of leaf epidermis wax, and the susceptible varieties did not change significantly. Among the different resistant varieties (lines), the contents of the other wax components were significantly different except for the content of ester components. Correlation analysis showed that there was a significant negative correlation between the contents of esters and the disease index in the waxy components. There was a significant positive correlation between the contents of alcohols and ketones and the disease index, while the other components and waxiness had no significant correlation with the disease index . Resistant varieties of leaf epidermal wax in the higher proportion of alkanes and esters, ketones and susceptible varieties of higher proportion. The results of scanning electron microscopy showed that the waxy crystals of the disease-resistant cultivars were mainly granular, rod-shaped and filamentous, while the irregular-shaped crystals accounted for a large proportion of the waxy crystals of susceptible cultivars. These results indicate that the composition and structure of the epidermal wax in rape leaves may be one of the mechanisms by which the resistant varieties resist and delay the invasion of pathogenic bacteria.