论文部分内容阅读
0 引言 乙型肝炎是一种常见传染病 ,危害极大 .我们通过PCR及基因重组技术将 adr亚型乙型肝炎病毒表面抗原HBs Ag基因克隆到腺病毒穿梭载体 ,构建了重组 HBs Ag腺病毒穿梭载体 ,为制备 HBs Ag腺病毒疫苗的研制打下了基础 .1 材料和方法1.1 材料 模板 p 1.2 (含 HBV adr亚
0 Introduction Hepatitis B is a common infectious disease, which is extremely harmful.We cloned the adr subtype HBsAg gene of hepatitis B virus surface into the adenovirus shuttle vector by PCR and gene recombination technology, and constructed the recombinant HBsAg adenovirus Shuttle vector, laid the foundation for the preparation of HBsAg adenovirus vaccine.1 Materials and methods 1.1 Material template p 1.2 (including HBV adr Asia