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目的研究白细胞介素13(IL-13)基因多态性与慢性阻塞性肺病(COPD)患者肺功能的关系。方法采用聚合酶链反应--限制性酶切片段长度多态性(PCR-RFLP)方法检测92例COPD患者IL-13基因+2044位点单核苷酸多态性,测定COPD患者肺功能,分析IL-13基因+2044位点多态性与肺功能的关系。结果 IL-13基因+2044位点基因型频率为G/G型48.9%、G/A型45.7%、A/A型5.4%,G/G型患者肺功能FEV1和FEV1/FVC(%)明显低于G/A型和A/A型(P<0.05)。结论 IL-13基因+2044位点多态性与COPD患者肺功能有关,G/G型COPD患者肺功能损害更加严重。
Objective To investigate the relationship between interleukin-13 (IL-13) gene polymorphism and lung function in patients with chronic obstructive pulmonary disease (COPD). Methods The single nucleotide polymorphisms of IL-13 gene +2044 in 92 patients with COPD were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The lung function, Analysis of IL-13 gene +2044 polymorphism and lung function relationship. Results The frequency of IL-13 gene +2044 locus was 48.9% in G / G type, 45.7% in G / A type and 5.4% in A / A type, and pulmonary function FEV1 and FEV1 / FVC (%) were significantly Lower than G / A type and A / A type (P <0.05). Conclusion Polymorphism of +2044 locus in IL-13 gene is associated with lung function in COPD patients, and lung function impairment is more serious in G / G COPD patients.