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高空间分辨率的共聚焦显微拉曼光谱技术从分子水平上提供了生物分子成分和结构的指纹性信息。以鼻咽癌细胞株C666-1、CNE2和正常鼻咽细胞株NP69为对象,利用拉曼光谱探索和分析癌细胞株与正常细胞株之间的差别。结果表明:谱峰比I1449/I1657(1.10)可以准确地将鼻咽癌与正常鼻咽细胞株分成两组,这一结论与相关支气管癌组织的报道结果一致;此外,采用主成分分析方法说明3种细胞株的拉曼光谱之间存在统计学差异,并以线性判别分析方法对其进行统计划分,灵敏度和特异性分别达到90%和100%。这些结论可以为拉曼光谱技术用于NPC的临床诊断提供依据,并有可能为鼻咽癌的早期诊断提供有益参考。
Confocal Raman microscopy with high spatial resolution provides molecular information on the molecular composition and structure of biomolecules. Nasopharyngeal carcinoma cell lines C666-1, CNE2 and normal nasopharyngeal cell line NP69 were studied by Raman spectroscopy to explore and analyze the differences between cancer cell lines and normal cell lines. The results showed that the peak-to-peak ratio I1449 / I1657 (1.10) could be used to classify nasopharyngeal carcinoma and normal nasopharyngeal carcinoma cell lines into two groups accurately. This conclusion is consistent with the reported results of bronchial carcinoma. In addition, using principal component analysis There were statistical differences among the three cell lines in Raman spectra, and they were statistically divided by linear discriminant analysis with sensitivity and specificity of 90% and 100% respectively. These conclusions can provide the basis for the clinical diagnosis of NPC by Raman spectroscopy and may provide a useful reference for the early diagnosis of nasopharyngeal carcinoma.