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采用人工催产和干法授精技术,在ZOOM645S解剖镜下对花(Hemibarbus maculatus)(♀)×唇(H.labeo)(♂)杂交子一代(F1)胚胎发育过程中各发育时期的发育时序和形态特征进行了观察。结果表明,受精卵为强黏性卵,卵径为1.60～1.80 mm。在孵化水温为(20±1)℃条件下,受精后1 h胚盘隆起;1 h20 min进入卵裂期;6 h 20 min进入桑椹期;8 h 20 min进入囊胚期;13 h 10 min进入原肠期;16 h 20min进入神经胚期;受精后18 h进入胚孔封闭期;21 h 30 min进入肌节出现期;27 h 20 min进入眼基出现期;28 h 40 min进入眼囊期;30 h 40 min进入尾芽期;32 h 30 min进入晶体出现期;34 h 40 min进入肌肉效应期;36 h 40 min进入耳石出现期;44 h进入心跳期;53 h 40 min进入眼色素形成期;60 h 40min进入血液循环期;68 h 30 min后开始出膜。杂交F1胚胎发育时序及形态特征均偏向母本花。
Using artificial ovulation and dry-fertilization techniques, the developmental timing and developmental stages of embryos in Hemibarbus maculatus (♀) × lip (♂) hybrid (F1) embryo during ZOOM645S dissection were studied. Morphological characteristics were observed. The results showed that the fertilized egg was a strong sticky egg with an egg diameter of 1.60-1.80 mm. The blastocyst uplift occurred 1 h after fertilization when the temperature of hatching water was (20 ± 1) ℃; the cleavage stage was reached at 1 h 20 min; the mulching stage was at 6 h 20 min; the blastocyst stage was at 8 h 20 min; the blastocyst stage was at 13 h 10 min Into the original intestine; 16 h 20min into the neural embryo; 18 h after fertilization into the embryo closure period; 21 h 30 min into the sarcomere stage; 27 h 20 min into the eye base period; 28 h 40 min into the eye capsule Period; 30 h 40 min into the tail bud stage; 32 h 30 min into the crystal appearance period; 34 h 40 min into the muscle effect period; 36 h 40 min into the otolith occurrence period; 44 h into the heart beat period; 53 h 40 min into the Eye pigment formation period; 60 h 40min into the blood circulation period; 68 h 30 min after the beginning of the film. F1 hybrid embryonic development timing and morphological characteristics are biased toward female flowers.