论文部分内容阅读
目的研究不同浓度血小板衍生因子-BB(PDGF-BB)对喉癌Hep2细胞增殖侵袭的影响及黏着斑激酶(FAK)、磷酸化黏着斑激酶397(FAKpY397)、基质金属蛋白酶2(MMP2)、基质金属蛋白酶9(MMP9)蛋白表达的变化。方法不同浓度(0 ng/mL、1 ng/mL、20 ng/mL、100 ng/mL)PDGF-BB诱导人喉癌Hep2细胞株,westernblot方法检测FAK、FAKpY397、MMP2、MMP9表达变化,MTT法检测细胞增殖,侵袭实验检测诱导后细胞侵袭能力的变化。结果 MTT示诱导后Hep2细胞增殖,在20 ng/mL浓度时达到最高,为(0.488±0.133),与对照组比较差异有统计学意义(P<0.05)。侵袭细胞数在各组均有增加,在20 ng/mL组达高峰为(22.75±1.42),较对照组差异有统计学意义(P<0.05),FAK、FAKpY397、MMP9、MMP2在20 ng/mL浓度时表达上调。结论 PDGF-BB上调Hep2细胞中FAK、FAKpY397、MMP9及MMP2表达,促进Hep2细胞增殖侵袭能力增强。
Objective To investigate the effects of different concentrations of platelet-derived factor-BB (PDGF-BB) on proliferation and invasion of laryngeal carcinoma Hep2 cells and the effects of focal adhesion kinase (FAK), phosphorylated focal adhesion kinase 397 (FAKpY397), matrix metalloproteinase 2 Metalloproteinase 9 (MMP9) protein expression changes. Methods Human laryngeal carcinoma Hep2 cells were induced by PDGF-BB at different concentrations (0 ng / mL, 1 ng / mL, 20 ng / mL and 100 ng / mL). The expressions of FAK, FAKpY397, MMP2 and MMP9 were detected by Western blot. MTT assay Detection of cell proliferation, invasion assay to detect changes in cell invasive ability. Results MTT showed that the proliferation of Hep2 cells reached the highest at 20 ng / mL (0.488 ± 0.133), which was significantly different from that of the control group (P <0.05). The number of invasive cells increased in all groups, reaching the peak at 20 ng / mL (22.75 ± 1.42), which was significantly lower than that of the control group (P <0.05). FAK, FAKpY397, MMP9 and MMP2 were at 20 ng / mL concentration increased expression. Conclusion PDGF-BB up-regulates the expression of FAK, FAKpY397, MMP9 and MMP2 in Hep2 cells and enhances the proliferation and invasion of Hep2 cells.