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目的:探讨食管鳞状细胞癌(esophageal squamous cell cancer,ESCC)患者的食管鳞癌组织、癌旁组织中Bin1基因启动子甲基化状态及其mRNA的表达及其临床意义。方法:采用实时荧光定量PCR(qRT-PCR)分别检测58例经病理证实的ESCC患者的食管鳞癌组织、癌旁组织中Bin1基因mRNA的表达情况;用甲基化特异性PCR(MSP)检测上述食管鳞癌组织中Bin1基因启动子甲基化状态,比较ESCC患者Bin1甲基化状态与临床病理分期的关系。结果:ESCC组织中Bin1基因启动子甲基化率明显高于癌旁组织(58.62%vs 25.86%,χ~2=12.76,P<0.01),Bin1甲基化状态与患者TNM分期、肿瘤侵润深度、分化程度、淋巴结转移相关(均P<0.05)。ESCC组织中Bin1 mRNA的表达水平明显低于癌旁组织[(0.78±0.05)vs(1.03±0.03),t=9.643,P<0.01)];发生Bin1甲基化的组织中Bin1 mRNA表达水平明显低于未发生甲基化的组织[(0.68±0.04)vs(0.85±0.07),t=2.476,P<0.05]。结论:Bin1基因启动子区甲基化状态可能与ESCC的发生密切相关,它是ESCC中Bin1 mRNA低表达或缺失的机制之一,且与ESCC进展和淋巴结转移有关。
Objective: To investigate the methylation status of Bin1 gene and its mRNA expression in esophageal squamous cell carcinoma (ESCC) and its adjacent tissues, and its clinical significance. Methods: The mRNA expression of Bin1 was detected by real-time quantitative PCR (qRT-PCR) in 58 esophageal squamous cell carcinoma tissues and paracancerous tissues confirmed by pathology. Methylation-specific PCR (MSP) The methylation status of Bin1 promoter in esophageal squamous cell carcinoma tissues was compared between Bin1 methylation status and clinical stage in ESCC patients. Results: The promoter methylation of Bin1 gene in ESCC tissues was significantly higher than that in paracancer tissues (58.62% vs 25.86%, χ ~ 2 = 12.76, P <0.01). The methylation status of Bin1 was correlated with TNM stage, Depth, differentiation, lymph node metastasis (all P <0.05). The expression level of Bin1 mRNA in ESCC tissues was significantly lower than that in paracancer tissues [(0.78 ± 0.05) vs (1.03 ± 0.03), t = 9.643, P <0.01) (0.68 ± 0.04) vs (0.85 ± 0.07), t = 2.476, P <0.05]. CONCLUSION: The methylation status of Bin1 gene promoter may be closely related to the occurrence of ESCC. It is one of the mechanisms of low expression or deletion of Bin1 mRNA in ESCC, which is related to the progress of ESCC and lymph node metastasis.