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目的对结直肠癌根治术后复发转移诊断中血清蛋白质指纹谱的诊断价值进行探讨。方法选取行结直肠癌根治术患者84例,均展开2年随访,以有明确复发转移的40例患者为观察组,以无复发转移的44例患者为对照组,利用表面增强激光解吸离子化飞行时间质谱仪对两组患者手术前后血清标本中血清蛋白质指纹谱进行测定。结果 M/Z为7763的蛋白血清在结直肠癌根治术前表达有明显增高,同时在确定患者出现术后转移时表达仍有显著增高。结论经血清蛋白质指纹谱分析,M/Z是7763的血清蛋白可作为结直肠癌根治术后复发转移诊断中的标识蛋白,在术后复发转移检测中具有重要应用价值。
Objective To investigate the diagnostic value of serum protein fingerprints in the diagnosis of recurrence and metastasis after radical operation of colorectal cancer. Methods Totally 84 patients undergoing radical resection of colorectal cancer were enrolled in this study. All patients were followed up for 2 years. Forty patients with definite recurrence and metastasis were selected as the observation group and 44 patients without metastasis as control group. Surface enhanced laser desorption ionization Time-of-flight mass spectrometry was used to determine the serum protein fingerprinting in serum samples from two groups before and after surgery. Results The protein serum with M / Z of 7763 was significantly increased before radical resection of colorectal cancer, and the expression of the protein was still significantly increased after the metastasis was confirmed. Conclusion Serum protein with M / Z of 7763 can be used as a marker protein in the diagnosis of recurrence and metastasis of radical resection of colorectal cancer by serum protein fingerprinting. It is of great value in the detection of postoperative recurrence and metastasis.