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目的了解用生理盐水稀释后的精液是否会对精子形态学检查产生影响。方法 30例男性精液标本,分别采用直接涂片和生理盐水稀释后涂片,涂片染色后进行精子形态学分析,比较精子头部、颈部和中段以及尾部的变化。结果精液直接涂片与生理盐水稀释后涂片在正常精子百分比、头部缺陷精子百分比、颈部及中段缺陷精子百分比的数据上无显著性差异;在尾部缺陷精子百分比的数据上差异有显著性。结论精子形态学检查中应用生理盐水可能会对精子尾部的检查造成影响。
Objectives To determine if sperm diluted with saline will affect sperm morphology. Methods Thirty male semen samples were smeared directly with saline and normal saline respectively. Sperm morphological analysis was performed after smear staining. The changes of sperm head, neck, middle and tail were compared. Results There was no significant difference in the percentages of normal sperm, the percentage of sperm with head defects, the percentage of sperm between neck and midsection in sperm smear and saline diluted smear, and there was significant difference in the percentage of tail sperm . Conclusion The application of saline in sperm morphology examination may affect the sperm tail examination.